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Retention gaps are mainly used for focusing sample components when introducing a large (liquid) sample directly onto the column, whereas, guard columns are used to protect the analytical column from contamination. When using a retention gap system, the retention gap will also act as a guard column, but its primary function is to create a focusing effect. This article will look at how retention gaps are used with different modes of injection and how to cost effectively use guard columns.

 

This article was written by Dr. Dawn Watson (Technical Expert, Crawford Scientific)

Guard Column or Retention Gap?