GC Chromatographic Parameters

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GC Chromatographic Parameters

The most important thing in GC is to obtain the optimum resolution in the minimum time. Each of the important chromatographic parameters (resolution, retention factor, selectivity, efficiency, and asymmetry) are explained and illustrated with real life examples in this module.

At the end of this module you will be able to estimate resolution between peaks within a chromatogram, explain how resolution is influenced by retention factor, selectivity, and efficiency, recognize which of these factors should be improved to increase resolution within specific separations, develop strategies to efficiently optimize resolution – using the other chromatographic parameters, and suggest ways to carry out optimization and troubleshooting of separations in a practical context.

Topics include:

Chromatographic resolution
The resolution equation
Retention factor
How to change retention factor
Effect of retention factor on resolution
Selectivity (separation) factor
How to change selectivity
Effect of selectivity on resolution
Efficiency
How to change efficiency
Effect of efficiency on resolution
Peak asymmetry

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