UV-Visible Detectors

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The most commonly used detector for HPLC analysis is the UV-visible detector. The detector fulfills many of the criteria for an ideal detector. This module will detail the workings of UV-visible detectors, including variable wavelength and diode array detectors. Key detectors settings that should be optimized are fully detailed.

Topics include:

The flow cell
UV-visible detection - quantitation
External standard quantitation
Calibration curve
UV absorbance
Variable wavelength UV-visible detectors
Diode array detectors (DAD)
Diode array detectors spectra
Diode array detectors - bandwidth
Diode array detectors - slit width
Diode array detectors - response time
Diode array detectors - reference wavelength
Choosing sample and reference settings for DAD
DAD - peak suppression

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