Mobile Phase Optimization Strategies for Reversed Phase HPLC

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Mobile Phase Optimization Strategies for Reversed Phase HPLC

In this webcast, we will be looking at strategies for optimizing mobile phase composition in order to achieve the required resolution in the minimum amount of time, which is of course the primary goal of everyone undertaking method development. The chemistry of the aqueous and organic portions of the mobile phase will be studied, and we will discuss how we can use these components to affect retention and improve the selectivity and resolution within our separations.

Topics include:

Review of reversed phase retention mechanisms
Methanol or acetonitrile - which one is best?
Eluotropic strength - quick ways to reach the ideal
Changing solvent - useful tools and approaches
Which buffer, what strength?
Mobile phase pH - understand the effects
Optimizing pH vs. retention
Strategies for when all else fails

Mobile Phase Optimization Strategies for Reversed Phase HPLC

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