The aim of this unit is to introduce hydrophilic interaction liquid chromatography (HILIC) as a novel mode of chromatography. We will present both advantages and limitations of the HILIC separation mode. Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) will also be discussed as a new HPLC option for hydrophilic analytes. The concepts of ion exchange chromatography and its separation potential for certain ionic samples will be explored.

Upon completion of this unit, you will be able to understand the benefits and limitations of using HILIC for certain applications, and recognize some of the most important parameters that can be used to alter the separation in HILIC mode. You will be able to demonstrate an awareness of the benefits and limitations of HILIC over normal and reversed phase chromatography and understand the benefits and limitations of using ERLIC for certain hydrophilic samples. You will learn how to recognize the important parameters that can be used to alter the separation in an ERLIC separation as well as demonstrate an awareness of the limitations of the ERLIC separation mode.

Topics include: 

• Introduction
• General considerations
• Normal phase chromatography
• HILIC separation mechanisms
• Stationary phases
• Columns
• Eluent systems
• Buffers and additives
• Gradient
• ERLIC
• Ion exchange chromatography
• Anion exchange mixed-modes of separation
• ERLIC vs. HILIC