Troubleshooting Sensitivity Issues (HPLC-UV)

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Troubleshooting Sensitivity Issues (HPLC-UV)

This webcast discusses the impact of sensitivity on HPLC-UV methods. We will discuss what sensitivity is, how we measure it, how it is achieved, and some of the problems that result in issues with sensitivity.

Topics include:

Limit of detection and quantitation (LOD/LOQ) – including new USP definition
LOQ – low concentration standards
Developing methods with high sensitivity
Benchmarking sensitivity – every method is different
What causes poor sensitivity – high noise or low signal
Detector issues – lamp and flow cell issues
Poor signal
High noise

Troubleshooting Sensitivity Issues (HPLC-UV)

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