We begin with a general overview of LC-MS as an analysis technique, what the benefits are, and what sort of samples the technique is best suited to. 

Then moving on to a more in-depth look at the HPLC section of this hyphenated technique – we concentrate on common parts that make up a typical HPLC system and how they work together to deliver successful chromatographic separations. 

The session will conclude with a comprehensive overview of the three predominant mechanisms for generating gas-phase ions using atmospheric pressure ionization; atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), and electrospray ionization (ESI). We will discuss the similarities and differences between the methods, as well as which is best suited to the different sample types. 

This course is the first module in the Fundamentals of LC-MS Video Training Course. It is accredited by the Royal Society of Chemistry & American Chemical Society - Analytical Division. 

More about the Video Training Course 

This course has been designed for anyone who is either new to LC-MS or who wishes to expand their current knowledge and understanding of the technique as a whole. 

All aspects of the technology and system are covered - from an overview of a typical HPLC system and important considerations when combining this separation technique with detection by mass spectrometry, all the way through the various atmospheric ionization processes and the different mass analyzers that are available. The main focus is on fundamental concepts but important practical aspects and some basic troubleshooting will also be introduced. 

To access the rest of the course and much more, talk to our team.